RECOMT
Image
Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- RECOMT - Bulletin (36 KB)
- Bulk Quote
- Similar Products
RECOMT
Sigma
Thrombin CleanCleave™ Kit
Description
| Application | For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems. |
| Features and Benefits | • Fast, efficient cleavage in as little as 2 hours • Thrombin is covalently bound to agarose for easy removal. • The robust cleavage reaction is effective at temperatures from 4°C to 37 °C and over a wide range of pH and ionic strengths. • Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA • Thrombin-agarose is reusable. |
| Quantity | 200μl of a 50% slurry of thrombin-agarose cleaves >85% of 1mg of fusion protein. |
| Legal Information | CleanCleave is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
Components
| Standard component | 10 |
| Disposable Spin Column 1 each |
References
| Reference | Di-Cera, E., et al., Linkage between proton binding and amidase activity in human alpha-thrombin: effect of ions and temperature. Biochemistry 30, 7913-7924, (1991) Abstract |
